概述

  • 产品名称
    Anti-TRIP12抗体
  • 描述
    兔多克隆抗体to TRIP12
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 75 and 125 of human TRIP12 (NP_004229.1)

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 220 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
    • 数据库链接
    • 别名
      • KIAA0045 antibody
      • MGC138849 antibody
      • MGC138850 antibody
      • Thyroid hormone receptor interactor 12 antibody
      • Thyroid receptor interacting protein 12 antibody
      • TRIP 12 antibody
      see all

    图片

    • All lanes : Anti-TRIP12 antibody (ab86220) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 1.5 µg
      Lane 3 : HeLa whole cell lysate at 0.5 µg

      Developed using the ECL technique

      Predicted band size : 220 kDa


      Exposure time : 3 minutes
    • Detection of human TRIP12 in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    文献

    This product has been referenced in:
    • Mounir Z  et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . Read more (PubMed: 27183006) »

    See 1 Publication for this product

    客户评价及客户问答

    We do not have data for mouse reactivity for this TRIP12 antibody, ab86220. The amino acid sequence of the immunogen that generated the antibody is well-conserved in the mouse protein so cross-reaction is possible, though not experimentally confirmed.<...

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    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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