Anti-Triosephosphate isomerase抗体(ab96696)

概述

  • 产品名称
    Anti-Triosephosphate isomerase抗体
    参阅全部 Triosephosphate isomerase 一抗
  • 描述
    兔多克隆抗体to Triosephosphate isomerase
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 187-249 of Human Triosephosphate isomerase (NP_000356).

  • 阳性对照
    • WB: 293T, HeLaS3, HepG2 and Raji cell lysates; A431, H1299, whole cell lysates. IHC-P: SNU16 tissue

性能

应用

Our Abpromise guarantee covers the use of ab96696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100.

靶标

  • 相关性
    Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
  • 细胞定位
    Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
  • 数据库链接
  • 别名
    • epididymis secretory protein Li 49 antibody
    • HEL-S-49 antibody
    • MGC88108 antibody
    • TIM antibody
    • TPI 1 antibody
    • TPI antibody
    • TPI1 antibody
    • TPID antibody
    • Triose phosphate isomerase 1 antibody
    • Triose phosphate isomerase antibody
    • Triosephosphate isomerase 1 antibody
    • Triosephosphate isomerase antibody
    see all

图片

  • All lanes : Anti-Triosephosphate isomerase antibody (ab96696) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 27 kDa
  • ab96696, at a 1/100 dilution, staining Triosephosphate isomerase in paraffin embedded SNU16 by Immunohistochemical analysis.

文献

This product has been referenced in:
  • Rai V  et al. Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep 7:45732 (2017). Read more (PubMed: 28378827) »
  • Peng X  et al. Autophagy promotes paclitaxel resistance of cervical cancer cells: involvement of Warburg effect activated hypoxia-induced factor 1-a-mediated signaling. Cell Death Dis 5:e1367 (2014). WB ; Human . Read more (PubMed: 25118927) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Loading amount
20 µg
Specification
Skeletal Muscle
Gel Running Conditions
Non-reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Maria Insenser

Verified customer

提交于 Jan 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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