Anti-Triosephosphate isomerase抗体(ab28760)


  • 产品名称Anti-Triosephosphate isomerase抗体
    参阅全部 Triosephosphate isomerase 一抗
  • 描述
    山羊多克隆抗体to Triosephosphate isomerase
  • 特异性This antibody is expected to recognise both reported isoforms.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:


    , corresponding to C terminal amino acids 237-249 of Human Triosephosphate isomerase, according to NP_000356.

  • 阳性对照
    • Human liver lysate, HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab28760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/16000.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.


  • 相关性Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
  • 细胞定位Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
  • 数据库链接
  • 别名
    • epididymis secretory protein Li 49 antibody
    • HEL-S-49 antibody
    • MGC88108 antibody
    • TIM antibody
    • TPI 1 antibody
    • TPI antibody
    • TPI1 antibody
    • TPID antibody
    • Triose phosphate isomerase 1 antibody
    • Triose phosphate isomerase antibody
    • Triosephosphate isomerase 1 antibody
    • Triosephosphate isomerase antibody
    see all

Anti-Triosephosphate isomerase antibody 图像

  • Anti-Triosephosphate isomerase antibody (ab28760) at 0.01 µg/ml + human liver lysate 30µg total protein per lane

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining Triosephosphate isomerase with ab28760 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a Tris/EDTA buffer (pH 9).

Anti-Triosephosphate isomerase antibody (ab28760)参考文献

This product has been referenced in:
  • Ekman M  et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). Rat . Read more (PubMed: 24589856) »
  • Seyfried NT  et al. Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards. J Proteome Res 11:2721-38 (2012). Read more (PubMed: 22416763) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification HEK293
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Aug 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 10 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Oct 13 2010