概述

  • 产品名称Anti-TRIM9抗体
    参阅全部 TRIM9 一抗
  • 描述
    兔多克隆抗体to TRIM9
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 500-549 (DGLHFNSTYN ARVKAFNKTG VSPYSKTLVL QTSEGKALQQ YPSERELRGI) of Human TRIM9 (NP_443210).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab82654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

靶标

  • 相关性TRIM9 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase TRIM9 antibody
    • HGNC:16288 antibody
    • Homolog of rat RING finger Spring antibody
    • KIAA0282 antibody
    • RING finger protein 91 antibody
    • RNF 91 antibody
    • RNF91 antibody
    • SPRING antibody
    • TRIM 9 antibody
    • Tripartite motif containing 9 antibody
    • Tripartite motif containing protein 9 antibody
    • Tripartite motif protein 9 antibody
    see all

Anti-TRIM9 antibody 图像

  • Anti-TRIM9 antibody (ab82654) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 79 kDa
    Observed band size : 61 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,37 kDa. We are unsure as to the identity of these extra bands.

Anti-TRIM9 antibody (ab82654)参考文献

ab82654 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82654.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"