Anti-TRIM72抗体(ab83302)

概述

  • 产品名称Anti-TRIM72抗体
    参阅全部 TRIM72 一抗
  • 描述
    兔多克隆抗体to TRIM72
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 288 - 337 (LEELTFDPSS AHPSLVVSSS GRRVECSEQK APPAGEDPRQ FDKAVAVVAH) of human TRIM72 (NP_001008275).

  • 阳性对照
    • Fetal muscle lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.Can be blocked with Human TRIM72 peptide (ab111656). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

靶标

  • 功能Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
  • 细胞定位Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Anti-TRIM72 antibody 图像

  • Anti-TRIM72 antibody (ab83302) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa

Anti-TRIM72 antibody (ab83302)参考文献

ab83302 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Primary myoblasts of striated muscle)
Gel Running Conditions Non-reduced Denaturing (10%)
Loading amount 40 µg
Specification Primary myoblasts of striated muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"