概述

  • 产品名称Anti-TRIM72抗体
    参阅全部 TRIM72 一抗
  • 描述
    小鼠多克隆抗体to TRIM72
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-269 of Human TRIM72 (isoform 2)

  • 阳性对照
    • Liver tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab68061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 53 , 30 kDa.

靶标

  • 功能Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
  • 细胞定位Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Anti-TRIM72 antibody 图像

  • Anti-TRIM72 antibody (ab68061) at 1/500 dilution + Human liver tissue lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

    Predicted band size : 53 , 30 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TRIM72 antibody (ab68061) at 1/500 dilution

    Lane 1 : TRIM72 (aa1-269, isoform 2) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 53 , 30 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-TRIM72 antibody (ab68061)参考文献

ab68061 has not yet been referenced specifically in any publications.

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