概述

  • 产品名称
  • 描述
    山羊多克隆抗体to TRIM71
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Dog, Human
  • 免疫原

    Synthetic peptide: C-NKLESTISAVQ, corresponding to internal sequence amino acids 409-419 of Human TRIM71 (NP_001034200.1).

  • 阳性对照
    • NIH3T3 lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab99487 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab99487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). An approximately 100 kDa band was observed in lysates of the cell line NIH3T3. An additional band of unknown identity was also consistently observed at 14 kDa. This band was successfully blocked by incubation with the immunizing peptide.

靶标

  • 功能
    E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
  • 组织特异性
    Specifically expressed in testis.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰
    Autoubiquitinated.
  • 细胞定位
    Cytoplasm, P-body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610206G21Rik antibody
    • Abnormal cell LINeage LIN 41 antibody
    • AL022943 antibody
    • E3 ubiquitin-protein ligase TRIM71 antibody
    • Gm1127 antibody
    • Homolog of C. elegans Lin 41 antibody
    • LIN 41 antibody
    • LIN41 antibody
    • LIN41_HUMAN antibody
    • mlin 41 antibody
    • Protein lin 41 homolog antibody
    • Protein lin-41 homolog antibody
    • RGD1566388 antibody
    • Ripply2 antibody
    • TRIM 71 antibody
    • TRIM71 antibody
    • Tripartite motif containing 71 antibody
    • Tripartite motif-containing protein 71 antibody
    see all

图片

  • Anti-TRIM71 antibody (ab99487) at 0.5 µg/ml + NIH3T3 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 92 kDa

实验方案

文献

ab99487 has not yet been referenced specifically in any publications.

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