The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked polyubiquitination of TMEM173/STING, thereby playing a key role in innate immunity. TMEM173/STING 'Lys-63'-linked ubiquitination activates the production of type I interferon IFN-beta following detection of pathogen- and host-derived double-stranded DNA.
序列相似性Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 RING-type zinc finger.