Anti-TRIM5 delta抗体(ab24181)


  • 产品名称Anti-TRIM5 delta抗体
  • 描述
    兔多克隆抗体to TRIM5 delta
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5 delta

  • 阳性对照
    • Mouse brain cell lysate.



Our Abpromise guarantee covers the use of ab24181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 1 µg/ml.


  • 功能Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • 结构域The B box-type zinc finger domain and the coiled-coil domain contribute to the higher and low order multimerization respectively which is essential for restriction activity (PubMed:22482711).
    The B30.2/SPRY domain acts as a capsid recognition domain. Polymorphisms in this domain explain the observed species-specific differences among orthologs (PubMed:22482711).
    The RING-type zinc finger domain confers E3 ubiquitin ligase activity and is essential for retrovirus restriction activity, autoubiquitination and higher-order multimerization (PubMed:22482711).
  • 翻译后修饰Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus.
    Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation.
  • 细胞定位Cytoplasm > P-body. Closely associates with proteasomal subunits in cytoplasmic bodies (By similarity). Colocalizes with SQSTM1 in cytoplasmic bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EG667823 antibody
    • Gm8833 antibody
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • Tripartite motif containing 5 transcript variant iota antibody
    • Tripartite motif containing 5 transcript variant kappa antibody
    • Tripartite motif containing protein 5 antibody
    • Tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all

Anti-TRIM5 delta antibody 图像

  • Immunofluorescence of TRIM5 delta in mouse brain tissue with ab24181 at 10 ug/mL.

  • Anti-TRIM5 delta antibody (ab24181) at 2 µg/ml + Mouse brain lysate

    Predicted band size : 56 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    TRIM5 delta is a splice variant of TRIM5 thus the predicted MW of the splice variant differs to the predicted MW of TRIM5.
  • ab24181 at 1µg/ml staining TRIM delta in mouse brain tissue by IHC

Anti-TRIM5 delta antibody (ab24181)参考文献

ab24181 has not yet been referenced specifically in any publications.

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