概述

  • 产品名称Anti-TRIM5 alpha抗体
    参阅全部 TRIM5 alpha 一抗
  • 描述
    山羊多克隆抗体to TRIM5 alpha
  • 特异性This antibody is expected to recognise all three human isoforms.
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Cat, Human
  • 免疫原

    Synthetic peptide:

    ASGILVNVKEEVTC

    , corresponding to N terminal amino acids 2-15 of Human RNF88.

  • 阳性对照
    • Human placenta, uterus and tonsil lysates.
  • 常规说明Official Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.

性能

应用

Our Abpromise guarantee covers the use of ab4389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution. PubMed: 19741300

靶标

  • 功能Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • 结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • 翻译后修饰Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
  • 细胞定位Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all

Anti-TRIM5 alpha antibody 图像

  • Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • Ab4389 (1ug/ml) staining human RNF88 in human tonsil by Immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

    A) positive cells next to a secondary follicle

    B) positive cells below tonsillar epithelium.

    In paraffin embedded Human Tonsil, ab4389 stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre.

  • Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-TRIM5 alpha antibody (ab4389)参考文献

This product has been referenced in:
  • Singh R  et al. TRIM5a and TRIM22 are differentially regulated according to HIV-1 infection phase and compartment. J Virol 88:4291-303 (2014). Read more (PubMed: 24478420) »
  • Battivelli E  et al. Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms. J Virol 85:7828-35 (2011). WB ; Human . Read more (PubMed: 21632761) »

See all 6 Publications for this product

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