Anti-TRIM47抗体(ab72234)
Key features and details
- Rabbit polyclonal to TRIM47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRIM47抗体 -
描述
兔多克隆抗体to TRIM47 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human TRIM47.
Database link: Q96LD4 -
阳性对照
- Whole cell lysate from Hela and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72234于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 70, 80, 130 kDa (predicted molecular weight: 70 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 70, 80, 130 kDa (predicted molecular weight: 70 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
TRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells. -
细胞定位
Cytoplasm. Nucleus -
数据库链接
- Entrez Gene: 738407 Chimpanzee
- Entrez Gene: 618638 Cow
- Entrez Gene: 91107 Human
- Omim: 611041 Human
- SwissProt: Q96LD4 Human
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别名
- Gene overexpressed in astrocytoma protein antibody
- GOA antibody
- RING finger protein 100 antibody
see all
图片
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All lanes : Anti-TRIM47 antibody (ab72234) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 70 kDa
Observed band size: 70,80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation/ Western Blot of TRIM47.
Lane 1: ab72234 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72234 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72234 尚未被引用在任何文献中。