重组Anti-TRIM29抗体[EPR3494] (ab108627)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3494] to TRIM29
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRIM29抗体[EPR3494]
参阅全部 TRIM29 一抗 -
描述
兔单克隆抗体[EPR3494] to TRIM29 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, A431, Daudi, 293T, LnCaP, and JAR cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3494 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108627于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa. |
靶标
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功能
It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line. -
组织特异性
Expressed in placenta, prostate and thymus. -
序列相似性
Contains 1 B box-type zinc finger. -
翻译后修饰
Constitutively phosphorylated by PKC on serine/threonine in A431 cells. -
细胞定位
Cytoplasm. Colocalizes with intermediate filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 23650 Human
- Entrez Gene: 72169 Mouse
- Entrez Gene: 300656 Rat
- Omim: 610658 Human
- SwissProt: Q14134 Human
- SwissProt: Q8R2Q0 Mouse
- Unigene: 504115 Human
- Unigene: 273277 Mouse
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别名
- Ataxia telangiectasia group D associated protein antibody
- Ataxia telangiectasia group D-associated protein antibody
- ATDC antibody
see all
图片
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All lanes : Anti-TRIM29 antibody [EPR3494] (ab108627) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRIM29 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 66 kDaLanes 1-4: Merged signal (red and green). Green - ab108627 observed at 74 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108627 Anti-TRIM29 antibody [EPR3494] was shown to specifically react with TRIM29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265878 (knockout cell lysate ab257770) was used. Wild-type and TRIM29 knockout samples were subjected to SDS-PAGE. ab108627 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-TRIM29 antibody [EPR3494] (ab108627) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108627 被引用在 5 文献中.
- Liu G et al. ARID1B/SUB1-activated lncRNA HOXA-AS2 drives the malignant behaviour of hepatoblastoma through regulation of HOXA3. J Cell Mol Med 25:3524-3536 (2021). PubMed: 33683826
- Lei G et al. TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell. Can J Gastroenterol Hepatol 2021:8870907 (2021). PubMed: 33824865
- Deng X et al. E3 ubiquitin ligase TRIM29 promotes pancreatic cancer growth and progression via stabilizing Yes-associated protein 1. J Transl Med 19:332 (2021). PubMed: 34353343
- Yang Y et al. MicroRNA-122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in vitro. Mol Med Rep 17:8244-8252 (2018). PubMed: 29693120
- Xu W et al. RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells. Oncol Rep 36:1411-8 (2016). PubMed: 27430345