概述

  • 产品名称Anti-TRIM29抗体
    参阅全部 TRIM29 一抗
  • 描述
    山羊多克隆抗体to TRIM29
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide:

    C-EKDRIKSFTTNE

    , corresponding to internal sequence amino acids 295-306 of Human TRIM29 (NP_036233.2).

  • 阳性对照
    • Human Peripheral Blood Mononucleocytes lysates.

性能

应用

Our Abpromise guarantee covers the use of ab99486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa). An approximately 70 kDa band was observed in Human Peripheral Blood Mononucleocytes lysates. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

靶标

  • 功能It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
  • 组织特异性Expressed in placenta, prostate and thymus.
  • 序列相似性Contains 1 B box-type zinc finger.
  • 翻译后修饰Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
  • 细胞定位Cytoplasm. Colocalizes with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ataxia telangiectasia group D associated protein antibody
    • Ataxia telangiectasia group D-associated protein antibody
    • ATDC antibody
    • FLJ36085 antibody
    • TRI29_HUMAN antibody
    • TRIM 29 antibody
    • TRIM29 antibody
    • Tripartite motif containing 29 antibody
    • Tripartite motif containing protein 29 antibody
    • Tripartite motif protein 29 antibody
    • Tripartite motif protein TRIM29 antibody
    • Tripartite motif-containing protein 29 antibody
    see all

Anti-TRIM29 antibody 图像

  • Anti-TRIM29 antibody (ab99486) at 1 µg/ml + Human Peripheral Blood Lymphocytes lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 66 kDa
  • ab99486 (3.8µg/ml) staining of paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-TRIM29 antibody (ab99486)参考文献

ab99486 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99486.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"