概述

  • 产品名称Anti-TRIM29抗体
    参阅全部 TRIM29 一抗
  • 描述
    兔多克隆抗体to TRIM29
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 538 and 588 of human TRIM29 (NP_036233.2)

  • 阳性对照
    • HeLa whole cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab74193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
    • 组织特异性Expressed in placenta, prostate and thymus.
    • 序列相似性Contains 1 B box-type zinc finger.
    • 翻译后修饰Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
    • 细胞定位Cytoplasm. Colocalizes with intermediate filaments.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ataxia telangiectasia group D associated protein antibody
      • Ataxia telangiectasia group D-associated protein antibody
      • ATDC antibody
      • FLJ36085 antibody
      • TRI29_HUMAN antibody
      • TRIM 29 antibody
      • TRIM29 antibody
      • Tripartite motif containing 29 antibody
      • Tripartite motif containing protein 29 antibody
      • Tripartite motif protein 29 antibody
      • Tripartite motif protein TRIM29 antibody
      • Tripartite motif-containing protein 29 antibody
      see all

    Anti-TRIM29 antibody 图像

    • Detection of TRIM29 by Western Blot of Immunprecipitate.
      ab74193 at 1µg/ml staining TRIM29 in HeLa whole cell lysate immunoprecipitated using ab74193 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.
    • IHC image of ab74193 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74193, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-TRIM29 antibody (ab74193)参考文献

    ab74193 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab74193.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"