概述

  • 产品名称Anti-TRIM25抗体
    参阅全部 TRIM25 一抗
  • 描述
    小鼠多克隆抗体to TRIM25
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    TRIM25 full-length Human protein ((AAH16924.1)

  • 阳性对照
    • Hela NE lysate, HeLa cell and TRIM25 transfected 293T cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab88669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
  • 组织特异性Ubiquitous.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • 结构域The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
    The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
  • 翻译后修饰Auto-ISGylated.
  • 细胞定位Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin/ISG15 ligase TRIM25 antibody
    • EFP antibody
    • Estrogen responsive finger protein antibody
    • Estrogen-responsive finger protein antibody
    • RING finger protein 147 antibody
    • RNF 147 antibody
    • RNF147 antibody
    • TRI25 antibody
    • TRI25_HUMAN antibody
    • TRIM 25 antibody
    • Trim25 antibody
    • Tripartite motif containing 25 antibody
    • Tripartite motif containing protein 25 antibody
    • Tripartite motif protein TRIM25 antibody
    • Tripartite motif-containing protein 25 antibody
    • Tripartite motifcontaining25 antibody
    • Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody
    • Z147 antibody
    • Zinc finger protein 147 (estrogen responsive finger protein) antibody
    • Zinc finger protein 147 antibody
    • Zinc finger protein-147 antibody
    • ZNF 147 antibody
    • ZNF147 antibody
    see all

Anti-TRIM25 antibody 图像

  • Anti-TRIM25 antibody (ab88669) at 1 µg/ml + Hela nuclear extract lysate at 50 µg

    Predicted band size : 71 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TRIM25 antibody (ab88669) at 1 µg/ml

    Lane 1 : TRIM25 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 71 kDa
    Observed band size : 71 kDa
  • Immunofluorescence of purified ab88669 staining TRIM25 on HeLa cell (antibody concentration 10 ug/ml)

Anti-TRIM25 antibody (ab88669)参考文献

ab88669 has not yet been referenced specifically in any publications.

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