Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity. The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
Zinc finger protein 147 (estrogen responsive finger protein) antibody
Zinc finger protein 147 antibody
Zinc finger protein-147 antibody
ZNF 147 antibody
Western blot - TRIM25 antibody (ab86365)
All lanes : Anti-TRIM25 antibody (ab86365) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 71 kDa
Exposure time : 30 seconds
Immunoprecipitation - TRIM25 antibody (ab86365)
Detection of TRIM25 by Western Blot of Immunprecipitate.
ab86365 at 1µg/ml staining TRIM25 in HeLa whole cell lysate immunoprecipitated using ab86365 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
Hu J et al. A common polymorphism in the caspase recruitment domain of RIG-I modifies the innate immune response of human dendritic cells. J Immunol185:424-32 (2010).
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