概述

  • 产品名称Anti-Trim22抗体
    参阅全部 Trim22 一抗
  • 描述
    兔多克隆抗体to Trim22
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide from within a region of internal sequence amino acids 359-408 (DVSGKIAWIL GVHSKISSLN KRKSSGFAFD PSVNYSKVYS RYRPQYGYWV) of Human Trim22 (NP_006065)

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab86116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性Trim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms.
  • 细胞定位Cytoplasm. Nucleus. Nucleus speckle. Nucleus; Cajal body. Note: Localizes predominanltly into the nucleus, found in cytoplasm to some extent. Forms distinct nuclear bodies that undergo dynamic changes during cell cycle progression. Nuclear bodies start to form in the early G0/G1 phase but become speckle-like in the S-phase and completely dispersed in mitosis. 35% of TRIM22 nuclear bodies overlap or are found adjacent to Cajal bodies.
  • 数据库链接
  • 别名
    • 50 kDa stimulated trans acting factor antibody
    • GPSTAF50 antibody
    • RING finger protein 94 antibody
    • RNF94 antibody
    • Staf 50 antibody
    • STAF50 antibody
    • Stimulated trans acting factor (50 kDa) antibody
    • Stimulated Trans Acting Factor (homolog of Mouse Rpt 1 gene) antibody
    • Trim22 antibody
    • Tripartite binding motif 22 antibody
    • Tripartite motif containing protein 22 antibody
    • TTRIM22RIM22 antibody
    see all

Anti-Trim22 antibody 图像

  • Anti-Trim22 antibody (ab86116) at 1 µg/ml (5% skim milk/ PBS buffer) + trabsfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

Anti-Trim22 antibody (ab86116)参考文献

ab86116 has not yet been referenced specifically in any publications.

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