概述

  • 产品名称Anti-TRIM16抗体
    参阅全部 TRIM16 一抗
  • 描述
    兔多克隆抗体to TRIM16
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human TRIM16, NP_006461.3.

  • 阳性对照
    • Whole cell lysate from Hela, 293T and NIH 3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab72129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能May play a role in the regulation of keratinocyte differentiation.
  • 组织特异性Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EBBP antibody
    • Estrogen responsive B box protein antibody
    • Estrogen-responsive B box protein antibody
    • TRI16_HUMAN antibody
    • TRIM 16 antibody
    • TRIM16 antibody
    • Tripartite motif containing 16 antibody
    • Tripartite motif containing protein 16 antibody
    • Tripartite motif-containing protein 16 antibody
    see all

Anti-TRIM16 antibody 图像

  • All lanes : Anti-TRIM16 antibody (ab72129) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of TRIM16.
    Lane 1: ab72129 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72129 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 10 seconds.

Anti-TRIM16 antibody (ab72129)参考文献

ab72129 has not yet been referenced specifically in any publications.

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