概述

  • 产品名称
    Anti-TRIM14抗体
    参阅全部 TRIM14 一抗
  • 描述
    兔多克隆抗体to TRIM14
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 204-253 (SFEPVKSFFK GLVEAVESTL QTPLDIRLSP TNHAYSALKV SSPRLVSNRP) of Human TRIM14, isoform beta (NP_150090).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human fetal liver lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Inhibits the transcriptional activity of SPI1 in a dose-dependent manner.
  • 组织特异性
    Highest expression in liver; undetectable in skeletal muscle.
  • 序列相似性
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
  • 结构域
    The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 5830400N10Rik antibody
      • KIAA0129 antibody
      • pub antibody
      • TRI14_HUMAN antibody
      • TRIM14 antibody
      • tripartite motif protein 14 antibody
      • tripartite motif protein TRIM14 antibody
      • tripartite motif-containing 14 antibody
      • Tripartite motif-containing protein 14 antibody
      see all

    图片

    • Western blot - TRIM14 antibody (ab85374)
      Western blot - TRIM14 antibody (ab85374)
      Anti-TRIM14 antibody (ab85374) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 28 kDa



      12% gel

    实验方案

    文献

    ab85374 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab85374？请让我们知道，以便我们可以引用本数据表中的参考文章。

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