- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-TRIM11抗体
参阅全部 TRIM11 一抗 -
描述兔多克隆抗体to TRIM11
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宿主Rabbit
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经测试应用适用于: IHC-P, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 1-155 of Human TRIM11.
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阳性对照
- 293T, A431, Jurkat and Raji whole cell lysates; Human Hepatoma tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab111694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
靶标
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功能E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.
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组织特异性Ubiquitous.
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通路Protein modification; protein ubiquitination.
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序列相似性Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
结构域The coiled-coil domain and the B30.2 domain are both necessary for interaction with HN and PAX6 (By similarity). They are also involved in MED15-binding.
The B30.2 domain may be involved cellular protein quality control by promoting the degradation of insoluble ubiquitinated proteins. -
细胞定位Cytoplasm. Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 81559 Human
- Entrez Gene: 338364 Mouse
- Entrez Gene: 94091 Mouse
- Entrez Gene: 360534 Rat
- Omim: 607868 Human
- SwissProt: Q96F44 Human
- SwissProt: Q99PQ2 Mouse
- SwissProt: B1H278 Rat
see all -
别名
- BIA 1 antibody
- BIA1 antibody
- E3 ubiquitin protein ligase TRIM11 antibody
see all
图片
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Western blot - Anti-TRIM11 antibody (ab111694)Anti-TRIM11 antibody (ab111694) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 53 kDa
7.5% SDS-PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM11 antibody (ab111694)ab111694 at 1/500 dilution staining TRIM11 in Formalin-fixed, paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.
文献
This product has been referenced in:
- Chen Y et al. Proliferation and invasion of ovarian cancer cells are suppressed by knockdown of TRIM11. Oncol Lett 14:2125-2130 (2017). Read more (PubMed: 28781653) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"