Triiodothyronine (T3) ELISA试剂盒(ab108685)

概述

  • 产品名称Triiodothyronine (T3) ELISA试剂盒
    参阅全部 Triiodothyronine (T3) 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 40 < 10.7%
    批次间
    样品 n Mean SD CV%
    Overall 3 < 9.1%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    0.05 ng/ml
  • 复检

    97.5 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.


     


    A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab108685 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Competitive ELISA Use at an assay dependent dilution.

    Triiodothyronine (T3) ELISA Kit 图像

    • Representative Standard Curve using ab108685

    实验方案

    Triiodothyronine (T3) ELISA Kit (ab108685)参考文献

    ab108685 has not yet been referenced specifically in any publications.

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