Anti-TRIB3抗体- Aminoterminal end (ab50516)


  • 产品名称Anti-TRIB3抗体- Aminoterminal end
    参阅全部 TRIB3 一抗
  • 描述
    兔多克隆抗体to TRIB3 - Aminoterminal end
  • 特异性This antibody is specific to TRIB3. The immunizing sequence has no identity to TRIB1 and TRIB2 isoforms.
  • 经测试应用适用于: WB, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:


    , corresponding to N terminal amino acids 11-29 of Human TRIB3 conjugated to KLH

  • 阳性对照
    • HEK-293 cells expressing human TRB3.



Our Abpromise guarantee covers the use of ab50516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide.
IHC-Fr 1/200.
IP Use at an assay dependent concentration.


  • 功能Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
  • 组织特异性Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C20orf97 antibody
    • Neuronal cell death inducible putative kinase antibody
    • Neuronal cell death-inducible putative kinase antibody
    • NIPK antibody
    • p65 interacting inhibitor of NF-kappaB antibody
    • p65-interacting inhibitor of NF-kappa-B antibody
    • SINK antibody
    • SKIP 3 antibody
    • SKIP3 antibody
    • TRB 3 antibody
    • TRB-3 antibody
    • TRB3 antibody
    • TRIB 3 antibody
    • Trib3 antibody
    • TRIB3_HUMAN antibody
    • Tribbles homolog 3 antibody
    • Tribbles pseudokinase 3 antibody
    • Tribbles3 antibody
    see all

Anti-TRIB3 antibody - Aminoterminal end 图像

  • All lanes : Anti-TRIB3 antibody - Aminoterminal end (ab50516) at 1/1000 dilution

    Lane 1 : HEK293 cells transfected with human TRIB3
    Lane 2 : HEK293 cells (untransfected)
    Lane 3 : HEK293 cells transfected with human TRIB3 with TRIB3 immunizing peptide at 20 µg/ml

    Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size : 40 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ab50516 staining TRIB3 in human brain tumor tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized 0.5% TX-100 before blocking with 10% serum for 1 hour 30 minutes at 250C. The sample was incubated with primary antibody (1/200) in 1% serum in PBS for 2 hours at 250C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

Anti-TRIB3 antibody - Aminoterminal end (ab50516)参考文献

This product has been referenced in:
  • Salazar M  et al. Cannabinoid action induces autophagy-mediated cell death through stimulation of ER stress in human glioma cells. J Clin Invest 119:1359-72 (2009). IP ; Human . Read more (PubMed: 19425170) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC in a PT link
Permeabilization No
Specification Muscle
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Sep 24 2015

Thank you for contacting us.  Both of these anti-TRIB3 antibodies has been tested for immunoprecipiation and are guaranteed for its use in this application. They have not been tested side by side and so we have no data on whether one may work better fo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela cells)
Total protein in input 500 µg
Specification Hela cells
Treatment cannabinoids (THC) for 8h
Immuno-precipitation step Protein G

Ms. María Salazar Roa

Verified customer

提交于 Aug 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (tumor xenografts from human glioma cells)
Specification tumor xenografts from human glioma cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX-100
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Mar Lorente

Verified customer

提交于 Jul 28 2009