概述

  • 产品名称Anti-TRIB3抗体
    参阅全部 TRIB3 一抗
  • 描述
    兔多克隆抗体to TRIB3
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TRIB3.

    (Peptide available as ab93788.)

  • 阳性对照
    • This antibody gave a positive signal in HepG2 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

靶标

  • 功能Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
  • 组织特异性Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C20orf97 antibody
    • Neuronal cell death inducible putative kinase antibody
    • Neuronal cell death-inducible putative kinase antibody
    • NIPK antibody
    • p65 interacting inhibitor of NF-kappaB antibody
    • p65-interacting inhibitor of NF-kappa-B antibody
    • SINK antibody
    • SKIP 3 antibody
    • SKIP3 antibody
    • TRB 3 antibody
    • TRB-3 antibody
    • TRB3 antibody
    • TRIB 3 antibody
    • Trib3 antibody
    • TRIB3_HUMAN antibody
    • Tribbles homolog 3 antibody
    • Tribbles pseudokinase 3 antibody
    • Tribbles3 antibody
    see all

Anti-TRIB3 antibody 图像

  • Anti-TRIB3 antibody (ab84174) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 140 kDa,250 kDa,57 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab84174 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84174, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, and MCF-7 cells at 1µg/ml.

Anti-TRIB3 antibody (ab84174)参考文献

ab84174 has not yet been referenced specifically in any publications.

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