Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (YPFHDIEPSS LFSKIRRGQF NIPETLSPKA KCLIRSILRR EPSERLTSQE) of Human TRIB2 (NP_067675).
Human fetal liver lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity.
Highly expressed in peripheral blood leukocytes.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain.
Cytoplasm. Cytoplasm > cytoskeleton. May associate with the cytoskeleton.
Information by UniProt
Western blot - TRIB2 antibody (ab84683)
Anti-TRIB2 antibody (ab84683) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
36 kDa (
why is the actual band size different from the predicted?
Gel concentration: 12%
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