Anti-TRIB1抗体- C-terminal (ab71267)

概述

  • 产品名称
    Anti-TRIB1抗体- C-terminal
    参阅全部 TRIB1 一抗
  • 描述
    兔多克隆抗体to TRIB1 - C-terminal
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human TRIB1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • T47D cell line lysate.

性能

应用

Our Abpromise guarantee covers the use of ab71267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能
    Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.
  • 组织特异性
    Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • 结构域
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C8FW antibody
    • G-protein-coupled receptor-induced gene 2 protein antibody
    • GIG-2 antibody
    • GIG2 antibody
    • SKIP1 antibody
    • TRB-1 antibody
    • TRB1 antibody
    • Trib1 antibody
    • TRIB1_HUMAN antibody
    • Tribbles homolog 1 antibody
    see all

图片

  • Anti-TRIB1 antibody - C-terminal (ab71267) at 1/60 dilution + T47D cell line lysate at 35 µg

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab71267 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
20 µg
Specification
macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

美弘 吉川

Verified customer

提交于 Aug 24 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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