Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TRIB1 with ab89021 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I and II cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TRIB1. Right - Merge.
Western blot - Anti-TRIB1 antibody (ab89021)
Predicted band size : 41 kDa Western blot analysis of COLO205 cell lysate labeling TRIB1 with ab89021 at 1.0µg/ml.
Western blot - TRIB1 antibody (ab89021)
Anti-TRIB1 antibody (ab89021) at 1 µg/ml + Human fetal intestine lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa Observed band size : 41 kDa Gel concentration: 12%