Anti-TRH Receptor抗体(ab72179)

概述

  • 产品名称
    Anti-TRH Receptor抗体
    参阅全部 TRH Receptor 一抗
  • 描述
    兔多克隆抗体to TRH Receptor
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human, Chimpanzee, Monkey
    预测可用于: Mouse, Rat, Cow, Opossum
  • 免疫原

    Synthetic peptide corresponding to a portion of amino acids 350-400 of Human TRH Receptor

  • 阳性对照
    • Human brain lysate. Human thyroid tissue.

性能

应用

Our Abpromise guarantee covers the use of ab72179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

图片

  • All lanes : Anti-TRH Receptor antibody (ab72179) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • ab72179 at 10µg/ml staining TRH Receptor in human thyroid by Immunohistochemistry using paraffin-embedded tissue.

文献

ab72179 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Olfactory Bulb)
Permeabilization
Yes - 1% triton, 30 minutes
Specification
Olfactory Bulb
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 30 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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