Anti-TRFP / MED20抗体(ab67800)

概述

  • 产品名称Anti-TRFP / MED20抗体
  • 描述
    小鼠多克隆抗体to TRFP / MED20
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length TRFP/ MED20 protein (Human)

  • 阳性对照
    • TRFP / MED20 transfected 293T cell lysate and Hela cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    NOTE: Product requires purification with Protein A prior to use in ICC/IF.

    WB: 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性TRFP / MED20 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
    • 细胞定位Nucleus
    • 数据库链接
    • 别名
      • MED20 antibody
      • Mediator complex subunit 20 antibody
      • TRF proximal protein homolog antibody
      • TRFP antibody

    Anti-TRFP / MED20 antibody 图像

    • All lanes : Anti-TRFP / MED20 antibody (ab67800) at 1/500 dilution

      Lane 1 : TRFP / MED20 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 23 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    • ab67800 at 10µg/ml staining TRFP/ MED20 in Hela cells.

    Anti-TRFP / MED20 antibody (ab67800)参考文献

    ab67800 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"