重组
RabMAb

Anti-TRF2抗体[EPR3518(2)] (ab108932)

概述

  • 产品名称
    Anti-TRF2抗体[EPR3518(2)]
    参阅全部 TRF2 一抗
  • 描述
    兔单克隆抗体[EPR3518(2)] to TRF2
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within Human TRF2.

  • 阳性对照
    • Jurkat and NAMALWA cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能
      Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
    • 组织特异性
      Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
    • 序列相似性
      Contains 1 HTH myb-type DNA-binding domain.
    • 结构域
      The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Telomeric DNA binding protein antibody
      • Telomeric DNA-binding protein antibody
      • Telomeric repeat binding factor 2 antibody
      • Telomeric repeat binding protein 2 antibody
      • Telomeric repeat-binding factor 2 antibody
      • TERF 2 antibody
      • Terf2 antibody
      • TERF2_HUMAN antibody
      • TRBF 2 antibody
      • TRBF2 antibody
      • TRF 2 antibody
      • TRF2 antibody
      • TTAGGG repeat binding factor 2 antibody
      • TTAGGG repeat-binding factor 2 antibody
      see all

    图片

    • All lanes : Anti-TRF2 antibody [EPR3518(2)] (ab108932) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : NAMALWA cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 56 kDa

    文献

    ab108932 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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