概述

  • 产品名称Anti-TRF2抗体
    参阅全部 TRF2 一抗
  • 描述
    山羊多克隆抗体to TRF2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Baculovirus expressed His-tagged whole length TRF2 protein was used for immunizing goat.

  • 阳性对照
    • Human cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab13589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use 2µg for 106 cells.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 60 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
    • 组织特异性Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
    • 序列相似性Contains 1 HTH myb-type DNA-binding domain.
    • 结构域The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Telomeric DNA binding protein antibody
      • Telomeric DNA-binding protein antibody
      • Telomeric repeat binding factor 2 antibody
      • Telomeric repeat binding protein 2 antibody
      • Telomeric repeat-binding factor 2 antibody
      • TERF 2 antibody
      • Terf2 antibody
      • TERF2_HUMAN antibody
      • TRBF 2 antibody
      • TRBF2 antibody
      • TRF 2 antibody
      • TTAGGG repeat binding factor 2 antibody
      • TTAGGG repeat-binding factor 2 antibody
      see all

    Anti-TRF2 antibody 图像

    • Immunoprecipitation (IP)/ Western Blot (WB) Analysis of TRF2 in human cell lysates. Lane 1. IP with TRF2 antibody (mouse ant-TRF2). Lane 2. IP with control mouse IgG. Lane 3. IP with TRF2 antibody (goat anti-TRF2). Lane 4. IP with pre-immune goat Ig. Lanes 1-4. WB with TRF2 antibody. TRF2 is detected as an ~ 66 kD protein.

    Anti-TRF2 antibody (ab13589)参考文献

    This product has been referenced in:
    • Wijeratne SS  et al. K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts. J Nutr Biochem 21:310-6 (2010). WB ; Human . Read more (PubMed: 19369050) »

    See 1 Publication for this product

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