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Our Abpromise guarantee covers the use of ab13579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 66 kDa.|
|ICC||Use at an assay dependent concentration.|
|IP||Use 2µg for 106 cells.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20679250|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab13579 staining TRF2 in bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.
Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.