Anti-TRF1抗体- ChIP Grade (ab1423)

概述

  • 产品名称Anti-TRF1抗体- ChIP Grade
    参阅全部 TRF1 一抗
  • 描述
    兔多克隆抗体to TRF1 - ChIP Grade
  • 特异性This antibody recognises TRF1. This antibody has not been tested against TFR2.
  • 经测试应用适用于: ChIP, WB, ICC/IF, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Human full length recombinant protein expressed in bacteria.

  • 阳性对照
    • HL60 WCE lysate

性能

应用

Our Abpromise guarantee covers the use of ab1423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent dilution. PubMed: 1946086
WB 1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
ICC/IF Use at an assay dependent dilution. PubMed: 20427319
IP Use at an assay dependent dilution.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
    • 组织特异性Highly expressed and ubiquitous. Isoform Pin2 predominates.
    • 序列相似性Contains 1 HTH myb-type DNA-binding domain.
    • 结构域The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
      The TRFH dimerization region mediates the interaction with TINF2.
    • 翻译后修饰Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
      ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
    • 细胞定位Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hTRF1 AS antibody
      • NIMA interacting protein 2 antibody
      • NIMA-interacting protein 2 antibody
      • PIN 2 antibody
      • PIN2 antibody
      • t TRF1 antibody
      • Telomeric protein Pin2 antibody
      • Telomeric protein Pin2/TRF1 antibody
      • Telomeric repeat binding factor (NIMA interacting) 1 antibody
      • Telomeric repeat binding factor 1 antibody
      • Telomeric repeat binding protein 1 antibody
      • Telomeric repeat-binding factor 1 antibody
      • TERF 1 antibody
      • Terf1 antibody
      • TERF1_HUMAN antibody
      • TRBF 1 antibody
      • TRBF1 antibody
      • TRF 1 antibody
      • TRF antibody
      • TTAGGG repeat binding factor 1 antibody
      • TTAGGG repeat-binding factor 1 antibody
      see all

    Anti-TRF1 antibody - ChIP Grade 图像

    • Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected.
    • Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.

    • The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

    Anti-TRF1 antibody - ChIP Grade (ab1423)参考文献

    This product has been referenced in:
    • Su CH  et al. An H2A Histone Isotype, H2ac, Associates with Telomere and Maintains Telomere Integrity. PLoS One 11:e0156378 (2016). ChIP ; Human . Read more (PubMed: 27228173) »
    • Root H  et al. FANCD2 limits BLM-dependent telomere instability in the alternative lengthening of telomeres pathway. Hum Mol Genet N/A:N/A (2016). Read more (PubMed: 27427384) »

    See all 18 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (8% resolution gel)
    Sample Human Cell lysate - whole cell (hepatocellular carcinoma)
    Specification hepatocellular carcinoma
    Treatment Inhibition of one gene's expression using lentiviral shRNA
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 16 2013

    Thank you for your reply.

    Our datasheet for this antibody does not suggest any specific ICC protocol, as a general ICC protocol should work with this antibody. The protocol stated on the datasheet belongs to a customer’s review who subm...

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    Thank you for contacting us and bringing this to our attention. The switch to PFA fixation and detergent permeabilization instead of methanol fixation that I mentioned in our conversation may help, as that is what has worked for customers in the past. ...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Breast carcinoma)
    Specification Breast carcinoma
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X-100
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 23 2009

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cell)
    Loading amount 100 µg
    Specification HeLa cell
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Geun Hyoung Ha

    Verified customer

    提交于 May 27 2009

    Application Western blot
    Sample Human Cell lysate - whole cell (Hela CELLS)
    Loading amount 50 µg
    Specification Hela CELLS
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Geun-Hyoung Ha

    Verified customer

    提交于 Apr 30 2009

    Thank you for this information, it is very useful and I can now really understand the protocol and the optimisation steps. I think the WB protocol looks very good (apart from my suggestion of running a high percentage gel), the IP protocol we sugge...

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    I have carefully looked at the protocol and unfortunately need more information to understand the problem, I am sorry for the delay. The antibody has not been tested on HeLa cells, it does cross react with human but it is known that cancer cells ca...

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    I'm sorry to hear that this antibody is not working out for you. If you send me the Abcam order number or purchase order number that was used for your order for ab1423, I can give you a refund. There is no need to return the product to us.

    1-10 of 16 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"