概述

  • 产品名称Anti-TREM2抗体
    参阅全部 TREM2 一抗
  • 描述
    山羊多克隆抗体to TREM2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    C-QVEHSTSRNQET

    , corresponding to internal sequence amino acids 154-165 of Mouse TREM2 (NP_112544.1).

  • 阳性对照
    • Mouse liver lysate IF/ICC: RAW 246.7 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).

靶标

  • 功能May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • 组织特异性Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • 疾病相关Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

Anti-TREM2 antibody 图像

  • Immunocytochemical immunoflurescence analysis of PFA-fixed human neuroblastoma cell line, labelling TREM2 using ab95470 at a dilution of 1/200 incubated for 2 hours and 24°C in 0.1M PBST with 10% donkey serum. Permeablisation was accomplished using 0.1M PBS with 1% Triton X. Blocking was done with 10% donkey serum in PBST and incubated for 30 minutes at 24°C. The secondary used was a polyclonal donkey anti-goat Alexa Fluor® 568 conjugate.

    See Abreview

  • ab95470 stained RAW 246.7 cells. The cells were 100% methanol fixed for 5 minutes at-20°C and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95470 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-TREM2 antibody (ab95470) at 0.3 µg/ml + Mouse liver lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.

Anti-TREM2 antibody (ab95470)参考文献

This product has been referenced in:
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . Read more (PubMed: 27662313) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Neuroblastoma cell line)
Permeabilization Yes - 0.1 M PBS with 1% Triton X
Specification Neuroblastoma cell line
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 20 2015

Application Western blot
Sample Human Serum (Human serum)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 20 µg
Specification Human serum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 20 2015

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