RabMAb

Anti-Trefoil Factor 3抗体[EPR3973] (ab109104)

概述

  • 产品名称Anti-Trefoil Factor 3抗体[EPR3973]
    参阅全部 Trefoil Factor 3 一抗
  • 描述
    兔单克隆抗体[EPR3973] to Trefoil Factor 3
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Trefoil Factor 3.

  • 阳性对照
    • Human uterus cancer and colon cancer lysates; Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
    • 组织特异性Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
    • 序列相似性Contains 1 P-type (trefoil) domain.
    • 细胞定位Secreted > extracellular space > extracellular matrix > apical lamina.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hITF antibody
      • hP1.B antibody
      • Intestinal trefoil factor antibody
      • ITF antibody
      • mITF antibody
      • OTTMUSP00000021729 antibody
      • P1B antibody
      • Polypeptide P1.B antibody
      • TFF3 antibody
      • TFF3_HUMAN antibody
      • TFI antibody
      • TREFOIL antibody
      • Trefoil factor (intestinal) antibody
      • Trefoil factor 3 antibody
      see all

    Anti-Trefoil Factor 3 antibody [EPR3973] 图像

    • All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution

      Lane 1 : Human uterus cancer lysate
      Lane 2 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 9 kDa
    • Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution.
    • Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)参考文献

    ab109104 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Serum (Mouse Serum)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification Mouse Serum
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
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    提交于 Jul 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"