概述

  • 产品名称Anti-TRAP80抗体
    参阅全部 TRAP80 一抗
  • 描述
    小鼠多克隆抗体to TRAP80
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Recombinant fusion protein (Fruit fly (Drosophila melanogaster))

  • 常规说明

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

应用

Our Abpromise guarantee covers the use of ab52885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 73 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in MED17 are the cause of microcephaly postnatal progressive with seizures and brain atrophy (MCPHSBA) [MIM:613668]. It is a disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth.
  • 序列相似性Belongs to the Mediator complex subunit 17 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 77-KD subunit antibody
      • Activator recruited cofactor 77 kDa component antibody
      • Activator-recruited cofactor 77 kDa component antibody
      • ARC77 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 6 antibody
      • cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa antibody
      • CRSP complex subunit 6 antibody
      • CRSP6 antibody
      • CRSP77 antibody
      • DRIP80 antibody
      • med17 antibody
      • MED17_HUMAN antibody
      • Mediator complex subunit 17 antibody
      • Mediator of RNA polymerase II transcription subunit 17 antibody
      • Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of antibody
      • Thyroid hormone receptor-associated protein complex 80 kDa component antibody
      • Thyroid hormone receptor-associated protein, 80-KD subunit antibody
      • Transcriptional coactivator CRSP77 antibody
      • Trap80 antibody
      • VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD antibody
      • Vitamin D3 receptor-interacting protein complex 80 kDa component antibody
      see all

    Anti-TRAP80 antibody 图像

    • All lanes : Anti-TRAP80 antibody (ab52885) at 1/1000 dilution

      Lane 1 : a total protein extract from E coli with 50ng of a Tagged irrelevant antigen (Negative control)
      Lane 2 : a total protein extract from E coli with 50ng of the antigen (Tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) HRP at 1/5000 dilution

      Predicted band size : 73 kDa

    Anti-TRAP80 antibody (ab52885)参考文献

    ab52885 has not yet been referenced specifically in any publications.

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