Anti-TRAP220/MED1 (phospho T1457)抗体- ChIP Grade (ab60950)

概述

  • 产品名称Anti-TRAP220/MED1 (phospho T1457)抗体- ChIP Grade
    参阅全部 TRAP220/MED1 一抗
  • 描述
    兔多克隆抗体to TRAP220/MED1 (phospho T1457) - ChIP Grade
  • 特异性ab60950 detects endogenous levels of TRAP220/MED1 only when phosphorylated at threonine 1457.
  • 经测试应用适用于: ELISA, ICC/IF, ChIP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human, African Green Monkey
  • 免疫原

    Synthetic phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457 (A-Y-TP-P-Q).

  • 阳性对照
    • Human heart tissue, HeLa cells and HuvEc cell extracts

性能

应用

Our Abpromise guarantee covers the use of ab60950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 19122196
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the Mediator complex subunit 1 family.
  • 翻译后修饰Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator-recruited cofactor 205 kDa component antibody
    • ARC205 antibody
    • CRSP1 antibody
    • CRSP200 antibody
    • DRIP205 antibody
    • DRIP230 antibody
    • MED1 antibody
    • MED1_HUMAN antibody
    • Mediator complex subunit 1 antibody
    • Mediator of RNA polymerase II transcription subunit 1 antibody
    • p53 regulatory protein RB18A antibody
    • PBP antibody
    • Peroxisome proliferator-activated receptor-binding protein antibody
    • PPAR binding protein antibody
    • PPAR-binding protein antibody
    • PPARBP antibody
    • PPARGBP antibody
    • RB18A antibody
    • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
    • Thyroid receptor-interacting protein 2 antibody
    • TR-interacting protein 2 antibody
    • Trap220 antibody
    • TRIP-2 antibody
    • TRIP2 antibody
    • Vitamin D receptor-interacting protein complex component DRIP205 antibody
    see all

Anti-TRAP220/MED1 (phospho T1457) antibody - ChIP Grade 图像

  • ab60950 at 1/50 - 1/100 dilution staining TRAP220/MED1 in human heart by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide
  • ab60950 at 1/500 - 1/1000 dilution staining TRAP220/MED1 in HeLa cells by Immunofluorescence.
  • All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody - ChIP Grade (ab60950) at 1/500 dilution

    Lane 1 : HuvEc cell extracts treated with Serum (20%, 30mins)
    Lane 2 : HuvEc cell extracts treated with Serum (20%, 30mins) with immunising peptide


    Predicted band size : 180 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)

Anti-TRAP220/MED1 (phospho T1457) antibody - ChIP Grade (ab60950)参考文献

This product has been referenced in:
  • Waters KM  et al. ERK oscillation-dependent gene expression patterns and deregulation by stress response. Chem Res Toxicol 27:1496-503 (2014). WB . Read more (PubMed: 25068892) »
  • Zhao W  et al. SIMPL enhancement of tumor necrosis factor-a dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function. PLoS One 8:e61123 (2013). WB, ChIP ; Human . Read more (PubMed: 23630580) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (VERO cells)
Specification VERO cells
Fixative Formaldehyde
Permeabilization Yes - Triton-X 0.3%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Fetal Lung Fibroblasts)
Specification Human Fetal Lung Fibroblasts
Fixative Formaldehyde
Permeabilization Yes - Triton-X 0.3%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"