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Synthetic peptide derived from internal region of human TRAP220/MED1
Our Abpromise guarantee covers the use of ab64965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).|
TRAP220/MED1 was immunoprecipitated from 500 µg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with 5 µg of ab64965 and Protein A/G magnetic beads. Westerm blot was performed from the immunoprecipitate using ab64965 at 1/1000 dilution.
Lane 1: U-2 OS whole cell lysate (input).
Lane 2: Negative control, no ab64965 present (-).
Lane 3: ab64965 IP in U-2 OS whole cell lysate.
Exposure time: 10 minutes
Exposure time: 10 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"