概述

  • 产品名称
    Anti-TRAP220/MED1抗体
    参阅全部 TRAP220/MED1 一抗
  • 描述
    兔多克隆抗体to TRAP220/MED1
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from internal region of human TRAP220/MED1

  • 阳性对照
    • Human breast carcinoma tissue. Extracts from Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab64965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).

靶标

  • 功能
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the Mediator complex subunit 1 family.
  • 翻译后修饰
    Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator-recruited cofactor 205 kDa component antibody
    • ARC205 antibody
    • CRSP1 antibody
    • CRSP200 antibody
    • DRIP205 antibody
    • DRIP230 antibody
    • MED1 antibody
    • MED1_HUMAN antibody
    • Mediator complex subunit 1 antibody
    • Mediator of RNA polymerase II transcription subunit 1 antibody
    • p53 regulatory protein RB18A antibody
    • PBP antibody
    • Peroxisome proliferator-activated receptor-binding protein antibody
    • PPAR binding protein antibody
    • PPAR-binding protein antibody
    • PPARBP antibody
    • PPARGBP antibody
    • RB18A antibody
    • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
    • Thyroid receptor-interacting protein 2 antibody
    • TR-interacting protein 2 antibody
    • Trap220 antibody
    • TRIP-2 antibody
    • TRIP2 antibody
    • Vitamin D receptor-interacting protein complex component DRIP205 antibody
    see all

图片

  • All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells, without immunising peptide
    Lane 2 : extracts from Jurkat cells, with immunising peptide


    Predicted band size : 180 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.

文献

This product has been referenced in:
  • Klümper N  et al. Mediator Complex Subunit MED1 Protein Expression Is Decreased during Bladder Cancer Progression. Front Med (Lausanne) 4:30 (2017). Read more (PubMed: 28367434) »
  • Jia Y  et al. Cardiomyocyte-Specific Ablation of Med1 Subunit of the Mediator Complex Causes Lethal Dilated Cardiomyopathy in Mice. PLoS One 11:e0160755 (2016). Read more (PubMed: 27548259) »

See all 7 Publications for this product

客户评价及客户问答

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
U2OS cells
Username

Abcam user community

Verified customer

提交于 Jul 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 28 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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