概述

  • 产品名称Anti-TRAP100抗体
    参阅全部 TRAP100 一抗
  • 描述
    小鼠多克隆抗体to TRAP100
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Fusion protein:

    EFLLKLTPLLDKADQRCNCDCTNFLLQECNKQGLLSEVNFASLVGKRTAD RDPQLKSSENANIQPNPGLILRAEPTVTNILKTMDADHSKSPEGLLGVLG

    , corresponding to amino acids 308/407 of Mouse TRAP100.

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

应用

Our Abpromise guarantee covers the use of ab21933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 110 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 组织特异性Ubiquitous. Abundant in skeletal muscle, heart and placenta.
  • 序列相似性Belongs to the Mediator complex subunit 24 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CRSP complex subunit 4 antibody
    • Activator recruited cofactor 100 kDa component antibody
    • Activator-recruited cofactor 100 kDa component antibody
    • ARC100 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 4 antibody
    • CRSP complex subunit 4 antibody
    • CRSP100 antibody
    • CRSP4 antibody
    • DRIP100 antibody
    • hTRAP100 antibody
    • KIAA0130 antibody
    • MED 24 antibody
    • MED24 antibody
    • MED24_HUMAN antibody
    • Mediator complex subunit 24 antibody
    • Mediator of RNA polymerase II transcription subunit 24 antibody
    • Mediator of RNA polymerase II transcription subunit 24 homolog antibody
    • MGC8748 antibody
    • OTTHUMP00000164458 antibody
    • OTTHUMP00000164460 antibody
    • THRAP4 antibody
    • Thyroid hormone receptor associated protein 4 antibody
    • Thyroid hormone receptor associated protein complex 100 kDa component antibody
    • Thyroid hormone receptor-associated protein 4 antibody
    • Thyroid hormone receptor-associated protein complex 100 kDa component antibody
    • Trap100 antibody
    • Trapp100 antibody
    • Vitamin D3 receptor interacting protein complex component DRIP100 antibody
    • Vitamin D3 receptor-interacting protein complex 100 kDa component antibody
    see all

Anti-TRAP100 antibody 图像

  • All lanes : Anti-TRAP100 antibody (ab21933) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 110 kDa

Anti-TRAP100 antibody (ab21933)参考文献

ab21933 has not yet been referenced specifically in any publications.

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