RabMAb

Anti-TRAP1抗体[EPR5381] (ab109323)

概述

  • 产品名称Anti-TRAP1抗体[EPR5381]
    参阅全部 TRAP1 一抗
  • 描述
    兔单克隆抗体[EPR5381] to TRAP1
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human TRAP1.

  • 阳性对照
    • WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/5000.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Chaperone that expresses an ATPase activity.
    • 组织特异性Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
    • 序列相似性Belongs to the heat shock protein 90 family.
    • 细胞定位Mitochondrion.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Heat shock protein 75 antibody
      • Heat shock protein 75 kDa antibody
      • Heat shock protein 75 kDa mitochondrial antibody
      • HSP 75 antibody
      • HSP 90L antibody
      • HSP75 antibody
      • HSP90L antibody
      • mitochondrial antibody
      • TNF receptor associated protein 1 antibody
      • TNFR associated protein 1 antibody
      • TNFR-associated protein 1 antibody
      • TRAP 1 antibody
      • TRAP-1 antibody
      • Trap1 antibody
      • TRAP1_HUMAN antibody
      • Tumor necrosis factor receptor associated protein antibody
      • Tumor necrosis factor type 1 receptor associated protein antibody
      • Tumor necrosis factor type 1 receptor-associated protein antibody
      see all

    Anti-TRAP1 antibody [EPR5381] 图像

    • All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 80 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    Anti-TRAP1 antibody [EPR5381] (ab109323)参考文献

    ab109323 has not yet been referenced specifically in any publications.

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