Anti-TRAP alpha抗体- Aminoterminal end (ab76595)

概述

  • 产品名称Anti-TRAP alpha抗体- Aminoterminal end
    参阅全部 TRAP alpha 一抗
  • 描述
    兔多克隆抗体to TRAP alpha - Aminoterminal end
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide N-terminal region of human SSR1 conjugated to KLH

  • 阳性对照
    • Transfected 293 cell lysates and human hepatocarcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/1000.
    IHC-P: 1/10 - 1/50.
    WB: 1/50 - 1/100. Detects bands of approximately 32 and 55 kDa (predicted molecular weight: 32 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
    • 序列相似性Belongs to the TRAP-alpha family.
    • 结构域Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.
    • 细胞定位Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp781N23103 antibody
      • FLJ14232 antibody
      • FLJ22100 antibody
      • FLJ23034 antibody
      • FLJ78242 antibody
      • FLJ93042 antibody
      • PGP35 antibody
      • Signal sequence receptor alpha antibody
      • Signal sequence receptor alpha subunit antibody
      • Signal sequence receptor subunit alpha antibody
      • SSR 1 antibody
      • SSR alpha antibody
      • SSR-alpha antibody
      • SSR1 antibody
      • SSRA_HUMAN antibody
      • SSRalpha antibody
      • Translocon associated protein alpha antibody
      • Translocon associated protein alpha subunit antibody
      • Translocon associated protein subunit alpha antibody
      • Translocon-associated protein subunit alpha antibody
      • TRAP a antibody
      • TRAP-alpha antibody
      • TRAPa antibody
      • TRAPalpha antibody
      see all

    Anti-TRAP alpha antibody - Aminoterminal end 图像

    • All lanes : Anti-TRAP alpha antibody - Aminoterminal end (ab76595) at 1/50 dilution

      Lane 1 : Non-transfected 293 cell lysates
      Lane 2 : TRAP alpha Transfected 293 cell lysates

      Lysates/proteins at 2 µg per lane.


      Predicted band size : 32 kDa
      Observed band size : 32,55 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab76595 at a 1/10 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

    Anti-TRAP alpha antibody - Aminoterminal end (ab76595)参考文献

    ab76595 has not yet been referenced specifically in any publications.

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    Thank you very much for your interest in ab76595.

    To our knowledge, ab76595 has not been tested in rabbit, but the rabbit and human sequences are highly homologous and we would expect the antibody to cross-react. Therefore, I can offer a dis...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"