概述

  • 产品名称Anti-TRAP alpha抗体
    参阅全部 TRAP alpha 一抗
  • 描述
    小鼠多克隆抗体to TRAP alpha
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human TRAP alpha protein (AAH07710.1).

  • 阳性对照
    • HeLa cell lysate. A-431 cell lysate. Cell lysate from transfected 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab88644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
  • 序列相似性Belongs to the TRAP-alpha family.
  • 结构域Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781N23103 antibody
    • FLJ14232 antibody
    • FLJ22100 antibody
    • FLJ23034 antibody
    • FLJ78242 antibody
    • FLJ93042 antibody
    • PGP35 antibody
    • Signal sequence receptor alpha antibody
    • Signal sequence receptor alpha subunit antibody
    • Signal sequence receptor subunit alpha antibody
    • SSR 1 antibody
    • SSR alpha antibody
    • SSR-alpha antibody
    • SSR1 antibody
    • SSRA_HUMAN antibody
    • SSRalpha antibody
    • Translocon associated protein alpha antibody
    • Translocon associated protein alpha subunit antibody
    • Translocon associated protein subunit alpha antibody
    • Translocon-associated protein subunit alpha antibody
    • TRAP a antibody
    • TRAP-alpha antibody
    • TRAPa antibody
    • TRAPalpha antibody
    see all

Anti-TRAP alpha antibody 图像

  • ab88644 detecting TRAP alpha in TRAP alpha-expressing 293 cells (Green) compared with negative control 293 cells (Black) by flow cytometry.
  • Anti-TRAP alpha antibody (ab88644) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : ~45 kDa. We are unsure as to the identity of these extra bands.
  • Anti-TRAP alpha antibody (ab88644) at 1 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-TRAP alpha antibody (ab88644) at 1 µg/ml

    Lane 1 : cell lysate from TRAP alpha transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa

Anti-TRAP alpha antibody (ab88644)参考文献

ab88644 has not yet been referenced specifically in any publications.

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