概述

  • 产品名称TRAP-6
  • 描述
    PAR1 agonist
  • 别名
    • Thrombin Receptor Activator Peptide 6
  • 生物学描述Peptide fragment (residues 42 - 47) of protease-activated receptor 1. PAR1 hexapeptide agonist. Stimulates transient mobilization of free intracellular calcium, platelet aggregation (EC50 = 0.8μM) and rapidly phosphorylates PDE3A.
  • 纯度> 96%

性能

    应用

    Our Abpromise guarantee covers the use of ab120801 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    TRAP-6 图像

    • ab6672 staining IL6 in serum starved PC3 cells treated with TRAP-6 (ab120801), by ICC/IF. Increase in expression of IL6 correlates with increased concentration of TRAP-6, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120801 (TRAP-6) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6672 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    TRAP-6 (ab120801)参考文献

    This product has been referenced in:
    • Picker SM  et al. Cell quality of apheresis-derived platelets treated with riboflavin-ultraviolet light after resuspension in platelet additive solution. Transfusion 52:510-6 (2012). Read more (PubMed: 21981030) »
    • Hunter RW  et al. Protein kinase C-mediated phosphorylation and activation of PDE3A regulate cAMP levels in human platelets. J Biol Chem 284:12339-48 (2009). Read more (PubMed: 19261611) »
    • Kaufmann R  et al. PAR 1-type thrombin receptors are involved in thrombin-induced calcium signaling in human meningioma cells. J Neurooncol 42:131-6 (1999). Read more (PubMed: 10421070) »
    • Vassallo RR  et al. Structure-function relationships in the activation of platelet thrombin receptors by receptor-derived peptides. J Biol Chem 267:6081-5 (1992). Read more (PubMed: 1313429) »

    See 0 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab120801.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"