Anti-Transportin 1抗体[D45] (ab10303)

概述

  • 产品名称Anti-Transportin 1抗体[D45]
    参阅全部 Transportin 1 一抗
  • 描述
    小鼠单克隆抗体[D45] to Transportin 1
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-P, ELISA, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human, Monkey
  • 免疫原

    Recombinant his-Transportin 1 (Human)

  • 常规说明


    Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Protein A purified from tissue culture supernatant.
  • Primary antibody说明Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.
  • 克隆单克隆
  • 克隆编号D45
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 107 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • 序列相似性Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Importin 2 antibody
    • Importin beta 2 antibody
    • Importin beta-2 antibody
    • IPO 2 antibody
    • IPO2 antibody
    • Karyopherin (importin) beta 2 antibody
    • Karyopherin beta 2 antibody
    • Karyopherin beta-2 antibody
    • KPN B2 antibody
    • KPNB 2 antibody
    • KPNB2 antibody
    • M9 interacting protein antibody
    • M9 region interacting protein antibody
    • M9 region interaction protein antibody
    • MIP 1 antibody
    • MIP antibody
    • MIP1 antibody
    • OTTHUMP00000126960 antibody
    • OTTHUMP00000222024 antibody
    • OTTHUMP00000226224 antibody
    • TNPO 1 antibody
    • Tnpo1 antibody
    • TNPO1_HUMAN antibody
    • Transportin 1 antibody
    • Transportin antibody
    • Transportin-1 antibody
    • Transportin1 antibody
    • TRN antibody
    see all

Anti-Transportin 1 antibody [D45] 图像

  • All lanes : Anti-Transportin 1 antibody [D45] (ab10303) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 107 kDa
    Observed band size : 107 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab10303 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10303, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab10303 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10303, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab10303 staining cultured P0 mouse neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% Triton X for 30 minutes  at 24°C. The cultured neurons were then stained with ab10303 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody.  Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    See Abreview

  • ICC/IF image of ab10303 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10303, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Transportin 1 antibody [D45] (ab10303)参考文献

This product has been referenced in:
  • Sharma P  et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015). WB . Read more (PubMed: 26403541) »
  • Darovic S  et al. Phosphorylation of C-terminal tyrosine residue 526 in FUS impairs its nuclear import. J Cell Sci 128:4151-9 (2015). Read more (PubMed: 26403203) »

See all 9 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neuronal culture)
Specification Neuronal culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (HeLa cytosolic extract)
Total protein in input 500 µg
Specification HeLa cytosolic extract
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount 50 µg
Specification HeLa cytosolic and nuclear extracts
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"