Anti-Transmembrane 4 L6 family member 1抗体(ab79958)

概述

  • 产品名称Anti-Transmembrane 4 L6 family member 1抗体
    参阅全部 Transmembrane 4 L6 family member 1 一抗
  • 描述
    兔多克隆抗体to Transmembrane 4 L6 family member 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Transmembrane 4 L6 family member 1.

    (Peptide available as ab98258.)

  • 阳性对照
    • This antibody gave a positive signal in Human ovarian carcinoma tissue lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab79958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).

靶标

  • 组织特异性Highly expressed in lung, breast, colon and ovarian carcinomas. It is also present on some normal cells, endothelial cells in particular.
  • 序列相似性Belongs to the L6 tetraspanin family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M3S1 antibody
    • Membrane component chromosome 3 surface marker 1 antibody
    • T4S1_HUMAN antibody
    • TAAL6 antibody
    • Tm4sf1 antibody
    • Transmembrane 4 L6 family member 1 antibody
    • Tumor-associated antigen L6 antibody
    see all

Anti-Transmembrane 4 L6 family member 1 antibody 图像

  • Anti-Transmembrane 4 L6 family member 1 antibody (ab79958) at 1/1 dilution + Human ovary tumor tissue lysate (carcinoma) - insoluble fraction (female, 74 years) (ab43987) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab79958 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79958, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Transmembrane 4 L6 family member 1 antibody (ab79958)参考文献

ab79958 has not yet been referenced specifically in any publications.

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