Detects endogenous levels of total translin protein.
Synthetic peptide derived from internal sequence of human translin.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150 mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/500 - 1/1000. Predicted molecular weight: 26 kDa.
Use at an assay dependent concentration.
Information by UniProt
Cellular Localisation: Cytoplasm. Nucleus
BCLF 1 antibody
Component 3 of promoter of RISC antibody
Western blot - Translin antibody (ab71775)
All lanes : Anti-Translin antibody (ab71775) at 1/1000 dilution Lane 1 : Jurkat cell lysate Lane 2 : Jurkat cell lysate with blocking peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Translin antibody (ab71775)
Immunohistochemical staining of paraffin-embedded
human brain tissue using translin antibody (ab71775) at 1/100 dilution. Staining performed in absence (left) and presence (right) of blocking peptide.
Immunocytochemistry/ Immunofluorescence-Translin antibody(ab71775)
ICC/IF image of ab71775 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71775, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"