Anti-Transketolase抗体(ab56199)

概述

  • 产品名称Anti-Transketolase抗体
    参阅全部 Transketolase 一抗
  • 描述
    兔多克隆抗体to Transketolase
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to C terminal residues of mouse Transketolase

性能

应用

Our Abpromise guarantee covers the use of ab56199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Transketolase antibody 图像

    • Anti-Transketolase antibody (ab56199) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 68 kDa
      Observed band size : 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 32 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 16 minutes

    Anti-Transketolase antibody (ab56199)参考文献

    ab56199 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (brain)
    Loading amount 15 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    提交于 Sep 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"