Anti-Transketolase抗体(ab56199)

概述

  • 产品名称
    Anti-Transketolase抗体
    参阅全部 Transketolase 一抗
  • 描述
    兔多克隆抗体to Transketolase
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to C terminal residues of mouse Transketolase

性能

应用

Our Abpromise guarantee covers the use of ab56199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • Anti-Transketolase antibody (ab56199) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 68 kDa
      Observed band size: 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 32 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 16 minutes


      The 74 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Transketolase.

    实验方案

    文献

    ab56199 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Loading amount
    15 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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