Anti-Transketolase抗体[7H1AA1] (ab112997)
Key features and details
- Mouse monoclonal [7H1AA1] to Transketolase
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Transketolase抗体[7H1AA1]
参阅全部 Transketolase 一抗 -
描述
小鼠单克隆抗体[7H1AA1] to Transketolase -
宿主
Mouse -
特异性
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
经测试应用
适用于: IHC-P, ICC/IF, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- HeLa and HepG2 whole cell lysate (ab7900); HL60 and HeLa cells. Human normal colon FFPE tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
ab112997 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
克隆
单克隆 -
克隆编号
7H1AA1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112997于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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序列相似性
Belongs to the transketolase family. - Information by UniProt
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数据库链接
- Entrez Gene: 7086 Human
- Omim: 606781 Human
- SwissProt: P29401 Human
- Unigene: 89643 Human
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别名
- EC 2.2.1.1 antibody
- FLJ34765 antibody
- TK antibody
see all
图片
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All lanes : Anti-Transketolase antibody [7H1AA1] (ab112997) at 1 µg/ml
Lane 1 : HepG2 Cell Lysate
Lane 2 : HeLa Cell Lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 68 kDa -
ab112997 at 1ug/ml staining Transketolase in HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
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ab112997 at 1ug/ml staining Transketolase in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI (blue). Non specific fluorescence was reduced using a signal enhancer.
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IHC image of Transketolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab112997 被引用在 6 文献中.
- Kraft AM & Petty HR Spatial locations of certain enzymes and transporters within preinvasive ductal epithelial cells predict human breast cancer recurrences. Am J Physiol Cell Physiol 319:C910-C921 (2020). PubMed: 32903032
- Zheng Y et al. Hepatitis B virus X protein-induced SH2 domain-containing 5 (SH2D5) expression promotes hepatoma cell growth via an SH2D5-transketolase interaction. J Biol Chem 294:4815-4827 (2019). PubMed: 30659097
- Tseng CW et al. Transketolase Regulates the Metabolic Switch to Control Breast Cancer Cell Metastasis via the a-Ketoglutarate Signaling Pathway. Cancer Res 78:2799-2812 (2018). PubMed: 29599405
- Burrai GP et al. A first immunohistochemistry study of transketolase and transketolase-like 1 expression in canine hyperplastic and neoplastic mammary lesions. BMC Vet Res 13:38 (2017). PubMed: 28143530
- Yang H et al. MicroRNA-497 regulates cisplatin chemosensitivity of cervical cancer by targeting transketolase. Am J Cancer Res 6:2690-2699 (2016). WB, IHC-P . PubMed: 27904781
- Croner RS et al. Identification of Predictive Markers for Response to Neoadjuvant Chemoradiation in Rectal Carcinomas by Proteomic Isotope Coded Protein Label (ICPL) Analysis. Int J Mol Sci 17:209 (2016). IHC . PubMed: 26861291