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RabMAb

Anti-Transglutaminase 2抗体[EP2957] (ab109200)

概述

  • 产品名称
    Anti-Transglutaminase 2抗体[EP2957]
    参阅全部 Transglutaminase 2 一抗
  • 描述
    兔单克隆抗体[EP2957] to Transglutaminase 2
  • 经测试应用
    适用于: WB, IP, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • U87-MG, A549 and HUVEC cell lysates; Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab109200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 序列相似性
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody [EP2957] 图像

  • All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 77 kDa
  • ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab109200 staining Transglutaminase 2 in mouse kidney and heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween-20 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS-T + 5% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

    See Abreview

Anti-Transglutaminase 2 antibody [EP2957] (ab109200)参考文献

ab109200 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Mouse Tissue sections (Kidney, Heart)
Specification
Kidney, Heart
Permeabilization
Yes - 0.1% Tween-20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 02 2014

Thank you for your enquiry.

I can confirm that ab109200 Transglutaminase 2 antibody [EP2957] has the following alignments to the isoforms of the protein:

Isoform 1 100%
isoform 2 and 3 26%

Therefore, the antibody i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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