重组Anti-Transglutaminase 2抗体[EP2957] (ab109200)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2957] to Transglutaminase 2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Transglutaminase 2抗体[EP2957]
参阅全部 Transglutaminase 2 一抗 -
描述
兔单克隆抗体[EP2957] to Transglutaminase 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: U87-MG, A549, HeLa and HUVEC cell lysates. IHC-P: Human kidney tissue. IHC-Fr: Mouse kidney and heart tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2957 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109200于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 77 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
序列相似性
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
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数据库链接
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Omim: 190196 Human
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
- Unigene: 517033 Human
- Unigene: 330731 Mouse
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别名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
图片
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All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGM2 knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-2: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TGM2 knockout HeLa cell lysate
Lane 3 : Wild-type A549 cell lysate
Lane 4 : TGM2 knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265245 (knockout cell lysate ab257085) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109200 was shown to recognize TGM2 in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109200 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa -
ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab109200 被引用在 11 文献中.
- Xia Y et al. Identification of a Diagnostic Signature and Immune Cell Infiltration Characteristics in Keloids. Front Mol Biosci 9:879461 (2022). PubMed: 35669563
- Sun W et al. The NEAT1_2/miR-491 Axis Modulates Papillary Thyroid Cancer Invasion and Metastasis Through TGM2/NF?b/FN1 Signaling. Front Oncol 11:610547 (2021). PubMed: 33738254
- Asad S et al. Proteomics-Informed Identification of Luminal Targets For In Situ Diagnosis of Inflammatory Bowel Disease. J Pharm Sci 110:239-250 (2021). PubMed: 33159915
- Strobl MAR et al. Mix and Match: Phenotypic Coexistence as a Key Facilitator of Cancer Invasion. Bull Math Biol 82:15 (2020). PubMed: 31953602
- Damaghi M et al. Collagen production and niche engineering: A novel strategy for cancer cells to survive acidosis in DCIS and evolve. Evol Appl 13:2689-2703 (2020). PubMed: 33294017