Anti-Transglutaminase 2抗体[CUB 7402] (ab2386)

概述

  • 产品名称Anti-Transglutaminase 2抗体[CUB 7402]
    参阅全部 Transglutaminase 2 一抗
  • 描述
    小鼠单克隆抗体[CUB 7402] to Transglutaminase 2
  • 经测试应用适用于: IHC-P, WB, ICC/IF, Flow Cyt, Othermore details
  • 种属反应性
    与反应: Mouse, Rabbit, Guinea pig, Cat, Dog, Human
  • 免疫原

    Purified guinea pig liver Transglutaminase 2.

  • 表位Epitope mapping for this antibody is amino acid 447-478
  • 阳性对照
    • Breast carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab2386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP Use at an assay dependent concentration.
IHC-P 1/50 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method. 10 mM citrate buffer, pH 6.0.
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19398782
Flow Cyt 1/10. (methanol fixed cells)



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Other Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 序列相似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody [CUB 7402] 图像

  • ab2386 staining human breat carcinoma by iHC-P.
  • ab2386 staining Transglutaminase 2 in mouse primary drmal fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 3% BSA in PBS + 0.1% Triton X100) for 16 hours at 4°C. An FITC-conjugated Goat monoclonal to mouse IgG, dilution 1/50, was used as secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

  • ab2386 staining breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.

  • Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

  • ab2386 staining Transglutaminase 2 in human SW480 colorectal carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in 1X PBS/ 1% BSA for 7 min at RT and permeabilized with 0.1% Triton-X100 in PBS for 5 min at RT. Samples were incubated with primary antibody (1/100: in 1X PBS / 1% BSA) for 20 minutes at 20°C. An FITC-conjugated rat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody. Slides counterstained with DAPI, and mounted with commercially available Prolong Slow-fade mounting medium.

    See Abreview

  • Immunohistochemical anaylsis of Human laryngeal squamous cell carcinoma tissue, staining Transglutaminase 2 with ab2386 at 1/100 dilution. Antigen retrieval was performed in citrate buffer at using a microwave oven. Staining was detected using DAB.

Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)参考文献

This product has been referenced in:
  • Yamaguchi M  et al. PLA2G5 regulates transglutaminase activity of human IL-4-activated M2 macrophages through PGE2 generation. J Leukoc Biol 100:131-41 (2016). Read more (PubMed: 26936936) »
  • Yang L  et al. GPR56 inhibits melanoma growth by internalizing and degrading its ligand TG2. Cancer Res 74:1022-31 (2014). Read more (PubMed: 24356421) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Specification Lung
Blocking step Casein in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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提交于 Oct 24 2016

Application Flow Cytometry
Sample Rhesus monkey Cell (rhesus monkey chorioretinal endothelial cell (RF/6)
Permeabilization Yes - 0.02% Triton X-­100 with 0.1mg/mL bovine serum albumin (BSA) for 7 min
Gating Strategy size and granularity
Specification rhesus monkey chorioretinal endothelial cell (RF/6
Preparation Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with 0.05% Trypsin-EDTA
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation Paraformaldehyde
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提交于 Aug 03 2015

Thank you for contacting us.

This clone has been published in many publications. You may find publications about the specificty of this clone e.g.

http://www.fasebj.org/content/suppl/2007/07/31/fj.06-7598com.DC1/06-7598SupplementalDat...

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Thank you for contacting us.

We have realized that there was an error on the datasheet which has now been corrected. The correct epitope mapping was from 447-478 amino acid instead of 451-454 which is a too small sequence. We apologize for thi...

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Thank you for your email.

The epitope mapping for this antibody is from amino acid 447 to 478. This is about 30 amino acids, which is largely sufficient for an antibody epitope. Please let me know if you still would have some concerns.
<...

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Thank you for contacting us.

The epitope mapping for this antibody is from amino acid 447 to 478.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Human SH-SY5Y neuroblatoma cells)
Loading amount 10 µg
Specification Human SH-SY5Y neuroblatoma cells
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Mrs. Adelheid Hanes

Verified customer

提交于 Nov 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (primary dermal fibroblasts from mouse)
Specification primary dermal fibroblasts from mouse
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% (v/v) Triton X100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SW480 colorectal carcinoma)
Specification SW480 colorectal carcinoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in PBS
Username

Ms. Doriana Cellura

Verified customer

提交于 Sep 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Duodenum)
Specification Duodenum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Feb 26 2008

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